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pcr amplified rest  (Addgene inc)


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    Structured Review

    Addgene inc pcr amplified rest
    Pcr Amplified Rest, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplified+rest/LPC-flag-REST-WT+(Plasmid+%2341903)/pm25403753-300-11-13
    Average 93 stars, based on 6 article reviews
    pcr amplified rest - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Over Expression:

    Article Title: Rapid neurogenesis through transcriptional activation in human stem cells
    Article Snippet: The cDNA-containing pENTR vectors were recombined using the LR reaction (Life Technologies) into customized lentiviral vectors based on FUW (Lois et al , ) containing a Gateway selection cassette (Life Technologies) called pLV_TRET_Ngn2-2A-Ngn1. .. The inducible REST overexpression vector was generated by replacing Ngn2-2A-Ngn1 by PCR-amplified REST (Addgene Plasmid 41903: LPC-flag-REST-WT, kind gift of Stephen Elledge) resulting in pLV_TRET_REST. .. The reverse tetracycline transactivator (rTA3) was PCR-amplified from pTRIPZ (Thermo Scientific) and cloned into a FUW lentiviral vector containing the human EF1α promoter.

    Article Title: Rapid neurogenesis through transcriptional activation in human stem cells.
    Article Snippet: The cDNA-containing pENTR vectors were recombined using the LR reaction (Life Technologies) into customized lentiviral vectors based on FUW (Lois et al, 2002) containing a Gateway selection cassette (Life Technologies) called pLV_TRET_Ngn2-2ANgn1. .. The inducible REST overexpression vector was generated by replacing Ngn2-2A-Ngn1 by PCR-amplified REST (Addgene Plasmid 41903: LPC-flag-REST-WT, kind gift of Stephen Elledge) resulting in pLV_TRET_REST. .. The reverse tetracycline transactivator (rTA3) Molecular Systems Biology 10: 760 | 2014 a 2014 The Authors D ow nloaded from https://w w w .em bopress.org on M arch 25, 2024 from IP 2401:4900:1ce0:5304:ec18:f025:b163:a1a2. was PCR-amplified from pTRIPZ (Thermo Scientific) and cloned into a FUW lentiviral vector containing the human EF1a promoter.

    Plasmid Preparation:

    Article Title: Rapid neurogenesis through transcriptional activation in human stem cells
    Article Snippet: The cDNA-containing pENTR vectors were recombined using the LR reaction (Life Technologies) into customized lentiviral vectors based on FUW (Lois et al , ) containing a Gateway selection cassette (Life Technologies) called pLV_TRET_Ngn2-2A-Ngn1. .. The inducible REST overexpression vector was generated by replacing Ngn2-2A-Ngn1 by PCR-amplified REST (Addgene Plasmid 41903: LPC-flag-REST-WT, kind gift of Stephen Elledge) resulting in pLV_TRET_REST. .. The reverse tetracycline transactivator (rTA3) was PCR-amplified from pTRIPZ (Thermo Scientific) and cloned into a FUW lentiviral vector containing the human EF1α promoter.

    Article Title: Rapid neurogenesis through transcriptional activation in human stem cells.
    Article Snippet: The cDNA-containing pENTR vectors were recombined using the LR reaction (Life Technologies) into customized lentiviral vectors based on FUW (Lois et al, 2002) containing a Gateway selection cassette (Life Technologies) called pLV_TRET_Ngn2-2ANgn1. .. The inducible REST overexpression vector was generated by replacing Ngn2-2A-Ngn1 by PCR-amplified REST (Addgene Plasmid 41903: LPC-flag-REST-WT, kind gift of Stephen Elledge) resulting in pLV_TRET_REST. .. The reverse tetracycline transactivator (rTA3) Molecular Systems Biology 10: 760 | 2014 a 2014 The Authors D ow nloaded from https://w w w .em bopress.org on M arch 25, 2024 from IP 2401:4900:1ce0:5304:ec18:f025:b163:a1a2. was PCR-amplified from pTRIPZ (Thermo Scientific) and cloned into a FUW lentiviral vector containing the human EF1a promoter.

    Generated:

    Article Title: Rapid neurogenesis through transcriptional activation in human stem cells
    Article Snippet: The cDNA-containing pENTR vectors were recombined using the LR reaction (Life Technologies) into customized lentiviral vectors based on FUW (Lois et al , ) containing a Gateway selection cassette (Life Technologies) called pLV_TRET_Ngn2-2A-Ngn1. .. The inducible REST overexpression vector was generated by replacing Ngn2-2A-Ngn1 by PCR-amplified REST (Addgene Plasmid 41903: LPC-flag-REST-WT, kind gift of Stephen Elledge) resulting in pLV_TRET_REST. .. The reverse tetracycline transactivator (rTA3) was PCR-amplified from pTRIPZ (Thermo Scientific) and cloned into a FUW lentiviral vector containing the human EF1α promoter.

    Article Title: Rapid neurogenesis through transcriptional activation in human stem cells.
    Article Snippet: The cDNA-containing pENTR vectors were recombined using the LR reaction (Life Technologies) into customized lentiviral vectors based on FUW (Lois et al, 2002) containing a Gateway selection cassette (Life Technologies) called pLV_TRET_Ngn2-2ANgn1. .. The inducible REST overexpression vector was generated by replacing Ngn2-2A-Ngn1 by PCR-amplified REST (Addgene Plasmid 41903: LPC-flag-REST-WT, kind gift of Stephen Elledge) resulting in pLV_TRET_REST. .. The reverse tetracycline transactivator (rTA3) Molecular Systems Biology 10: 760 | 2014 a 2014 The Authors D ow nloaded from https://w w w .em bopress.org on M arch 25, 2024 from IP 2401:4900:1ce0:5304:ec18:f025:b163:a1a2. was PCR-amplified from pTRIPZ (Thermo Scientific) and cloned into a FUW lentiviral vector containing the human EF1a promoter.

    Polymerase Chain Reaction:

    Article Title: Rapid neurogenesis through transcriptional activation in human stem cells
    Article Snippet: The cDNA-containing pENTR vectors were recombined using the LR reaction (Life Technologies) into customized lentiviral vectors based on FUW (Lois et al , ) containing a Gateway selection cassette (Life Technologies) called pLV_TRET_Ngn2-2A-Ngn1. .. The inducible REST overexpression vector was generated by replacing Ngn2-2A-Ngn1 by PCR-amplified REST (Addgene Plasmid 41903: LPC-flag-REST-WT, kind gift of Stephen Elledge) resulting in pLV_TRET_REST. .. The reverse tetracycline transactivator (rTA3) was PCR-amplified from pTRIPZ (Thermo Scientific) and cloned into a FUW lentiviral vector containing the human EF1α promoter.

    Article Title: Rapid neurogenesis through transcriptional activation in human stem cells.
    Article Snippet: The cDNA-containing pENTR vectors were recombined using the LR reaction (Life Technologies) into customized lentiviral vectors based on FUW (Lois et al, 2002) containing a Gateway selection cassette (Life Technologies) called pLV_TRET_Ngn2-2ANgn1. .. The inducible REST overexpression vector was generated by replacing Ngn2-2A-Ngn1 by PCR-amplified REST (Addgene Plasmid 41903: LPC-flag-REST-WT, kind gift of Stephen Elledge) resulting in pLV_TRET_REST. .. The reverse tetracycline transactivator (rTA3) Molecular Systems Biology 10: 760 | 2014 a 2014 The Authors D ow nloaded from https://w w w .em bopress.org on M arch 25, 2024 from IP 2401:4900:1ce0:5304:ec18:f025:b163:a1a2. was PCR-amplified from pTRIPZ (Thermo Scientific) and cloned into a FUW lentiviral vector containing the human EF1a promoter.



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    93
    Addgene inc pcr amplified rest
    Pcr Amplified Rest, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplified+rest/LPC-flag-REST-WT+(Plasmid+%2341903)/pm25403753-300-11-13
    Average 93 stars, based on 1 article reviews
    pcr amplified rest - by Bioz Stars, 2026-09
    93/100 stars
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